CST, 5251S, Phospho-TCTP (Ser46) Antibody

Polyclonal Antibody for studying TCTP (Ser46) phosphate. Validated for WB,IHC,IF,F. Highly specific and rigorously validated in-house, Phospho-TCTP (Ser46) Antibody (CST #5251) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:100 Immunofluorescence (Immunocytochemistry): 1:100 Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Phospho-TCTP (Ser46) Antibody recognizes endogenous levels TCTP protein only when phosphorylated at Ser46. In some cell types this antibody may recognize an 80-100 kDa protein of unknown origin. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser46 of human TCTP protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Translationally controlled tumor protein (TCTP/p23/HRF) is a ubiquitously expressed and highly conserved protein involved in various cellular processes, such as its role as a histamine releasing factor in chronic allergic disease (1). TCTP binds tubulin in a cell cycle dependent manner and is associated with the mitotic spindle (2). In addition, TCTP interacts with the actin cytoskeleton to regulate cell shape (3). In mitosis, TCTP is phosphorylated by PLK at Ser46, decreasing microtubule stability (4,5). TCTP interacts with the small GTPase Rheb, possibly acting as a GEF, thereby activating the TORC1 pathway and controlling cell growth and proliferation (6,7). TCTP has also been shown to be involved in apoptosis and cell stress (8-11). In cultured cells, reduction in TCTP expression can cause loss of the malignant phenotype (12). Alternate Names FLJ27337; Fortilin; Histamine-releasing factor; HRF; p02; p23; TCTP; TPT1; Translationally-controlled tumor protein; tumor protein, translationally-controlled 1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 23 SOURCE: Rabbit