CST, 52520S, Prostein/SLC45A3 (F5U4V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SLC45A3/P501s. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Prostein/SLC45A3 (F5U4V) Rabbit Monoclonal Antibody (CST #52520) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Prostein/SLC45A3 (F5U4V) Rabbit Monoclonal Antibody recognizes endogenous levels of total Prostein/SLC45A3 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Prostein/SLC45A3 protein. Background Solute carrier family 45 member 3 (SLC45A3), also known as prostate cancer-associated protein 6 (prostein), is an androgen-sensitive, multi-pass transmembrane sucrose transporter (1). SLC45A3 is highly expressed in both normal prostate and prostate cancers (1). SLC45A3 may have limited expression in white matter oligodendrocytes and epithelial cells of the kidney medullary collecting duct, where it regulates oligodendrocyte differentiation and urine osmolarity, respectively (2,3). In high-grade prostate cancers, there is a loss of SLC45A3 expression that corresponds with an increase in SLC45A3 5' protein fusions with multiple ETS-family proteins, including ERG fusions (4,5), PEA3-family (ETV1, ETV4, and ETV5) fusions (6), and ELK4 fusions (7). In ETS-negative prostate cancers, SLC45A3-BRAF fusions have been observed that are sensitive to RAF inhibitors (8). Alternate Names IPCA-2; IPCA-6; IPCA-8; IPCA6; p501s; PCANAP2; PCANAP6; PCANAP8; prostate cancer associated protein 2; prostate cancer associated protein 6; prostate cancer associated protein 8; prostate cancer-associated gene 2; prostate cancer-associated gene 6; prostate cancer-associated gene 8; Prostate cancer-associated protein 6; Prostein; PRST; S45A3; SLC45A3; Solute carrier family 45 member 3; solute carrier family 45, member 3 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 50 Source/Isotype: Rabbit IgG