CST, 52583S, NF-kappaB2 p100/p52 (D9S3M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NFkB-p100 mouse. Validated for WB,IP,C&R,C&T. Available in 2 sizes. Highly specific and rigorously validated in-house, NF-kappaB2 p100/p52 (D9S3M) Rabbit Monoclonal Antibody (CST #52583) is ready to ship. Product Usage Information The CUT&RUN dilution was determined using CUT&RUN Assay Kit # 86652 . The CUT&Tag dilution was determined using CUT&Tag Assay Kit # 77552 . Western Blotting: 1:1000 Immunoprecipitation: 1:200 CUT&RUN: 1:50 CUT&Tag: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, CUT&RUN, CUT&Tag Specificity / Sensitivity NF-kappaB2 p100/p52 (D9S3M) Rabbit Monoclonal Antibody recognizes endogenous levels of the p100 precursor and p52 active form of rodent NF-κB2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of mouse NF-κB2 p100/p52 protein. Background Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11). Alternate Names DNA-binding factor KBF2; lyt; NF kappaB2; NF-kappaB2; NFkB-p100; Nfkb2; Nuclear factor NF-kappa-B p100 subunit; Nuclear factor NF-kappa-B p52 subunit; nuclear factor of kappa light polypeptide gene enhancer in B cells 2, p49/p100; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2; nuclear factor of kappa light polypeptide gene enhancer in B-cells 2, p49/p100; p49; p49/p100; p50B; p52 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 120, 52 Source/Isotype: Rabbit IgG