CST, 5258S, Fetuin A (F180) Antibody

Polyclonal Antibody for studying FetuinA. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Fetuin A (F180) Antibody (CST #5258) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Fetuin A (F180) Antibody detects endogenous levels of total fetuin A protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe180 of human fetuin A protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Fetuin A is produced by liver and secreted to plasma, and selectively concentrated in bone matrix. It is synthesized as a precursor and processed to generate 2 chains that are held together by a disulfide bond. It is a systemically acting calcification inhibitor (1). Reduced level of fetuin A in serum is associated with increased cardiovascular mortality in dialysis patients (2,3). Fetuin A can inhibit insulin-induced tyrosine phosphorylation of the insulin receptor tyrosine kinase and insulin receptor substrate-1 (4). Fetuin A deficient mice show high insulin sensitivity and high levels of serum fetuin A are associated with insulin resistance in humans (5,6). Alternate Names A2HS; AHS; AHSG; alpha 2-HS glycoprotein; Alpha-2-HS-glycoprotein; Alpha-2-HS-glycoprotein chain A; Alpha-2-HS-glycoprotein chain B; Alpha-2-Z-globulin; APMR1; Ba-alpha-2-glycoprotein; FETUA; Fetuin-A; HSGA Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 45-50 SOURCE: Rabbit