CST, 5259S, PCM-1 (Q15) Antibody

Polyclonal Antibody for studying PCM-1. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, PCM-1 (Q15) Antibody (CST #5259) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:400 Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity PCM-1 (Q15) Antibody detects endogenous levels of total PCM-1 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PCM-1 protein. Antibodies were purified by protein A and peptide affinity chromatography. Background The centrosome is composed of a pair of centrioles surrounded by electron-dense pericentriolar material and functions as the microtubule-organizing center responsible for microtubule nucleation and spindle organization during cell cycle progression (1). Pericentriolar material 1 (PCM-1) is a large, 228 kDa protein associated with the centrosome in a cell cycle-dependent manner (2). PCM-1 localizes to small cytoplasmic granules called centriolar satellites (3). PCM-1 is required for the assembly of several centrosomal proteins, including centrin, pericentrin, ninein, NEK2, and CEP250 (4-8). Chromosomal translocations involving genes encoding PCM-1 and the tyrosine kinases Ret and Jak2 are associated with some cancers, including papillary thyroid carcinoma and myeloid leukemia (9-11). Alternate Names hPCM-1; PCM-1; PCM1; pericentriolar material 1; Pericentriolar material 1 protein; pericentriolar material 1, PCM1; PTC4; RET/PCM-1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 228 SOURCE: Rabbit