CST, 52631T, Phospho-14-3-3 zeta/delta (Ser58)/eta (Ser59)/gamma (Ser59)/beta/alpha (Ser60) (E6B3G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying 14-3-3 gamma (Ser59) phosphate/14-3-3 beta/alpha (Ser60) phosphate/14-3-3 eta (Ser59) phosphate/14-3-3 zeta/delta (Ser58) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-14-3-3 zeta/delta (Ser58)/eta (Ser59)/gamma (Ser59)/beta/alpha (Ser60) (E6B3G) Rabbit Monoclonal Antibody (CST #52631) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-14-3-3 zeta/delta (Ser58)/eta (Ser59)/gamma (Ser59)/beta/alpha (Ser60) (E6B3G) Rabbit Monoclonal Antibody detects endogenous levels of 14-3-3 ζ/δ only when phosphorylated at Ser58, 14-3-3 η and 14-3-3 γ only when phosphorylated at Ser59, and 14-3-3 α/β only when phosphorylated at Ser60. This antibody does not cross-react with 14-3-3 ε, 14-3-3 θ, or 14-3-3 σ. This antibody also recognizes a non-specific band of unknown origin at 80 kDa and 180 kDa. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser58 of human 14-3-3 ζ/δ protein. Background The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways (1,2). 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, γ, ε, σ, ζ, τ, and η that have been identified in mammals. The initially described α and δ isoforms are confirmed to be phosphorylated forms of β and ζ, respectively (3). Through their amino-terminal α helical region, 14-3-3 proteins form homo- or heterodimers that interact with a wide variety of proteins: transcription factors, metabolic enzymes, cytoskeletal proteins, kinases, phosphatases, and other signaling molecules (3,4). The interaction of 14-3-3 proteins with their targets is primarily through a phospho-Ser/Thr motif. However, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation independent interactions has been observed (4). 14-3-3 binding masks specific sequences of the target protein, and therefore, modulates target protein localization, phosphorylation state, stability, and molecular interactions (1-4). 14-3-3 proteins may also induce target protein conformational changes that modify target protein function (4,5). Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in development and in acute response to extracellular signals and drugs, suggesting that 14-3-3 isoforms may perform different functions despite their sequence similarities (4). Several studies suggest that 14-3-3 isoforms are differentially regulated in cancer and neurological syndromes (2,3). Phosphorylation of 14-3-3ζ at Ser58 may regulate dimerization and affect its ability to interact with partner proteins, including p53 and ASK1 (6,7). Alternate Names 14-3-3 alpha; 14-3-3 beta; 14-3-3 delta; 14-3-3 eta; 14-3-3 gamma; 14-3-3 protein beta/alpha; 14-3-3 protein beta/alpha, N-terminally processed; 14-3-3 protein eta; 14-3-3 protein gamma; 14-3-3 protein gamma, N-terminally processed; 14-3-3 protein zeta/delta; 14-3-3 protein/cytosolic phospholipase A2; 14-3-3 zeta; 14-3-3-zeta; 14-3-3GAMMA; 1433B; 1433F; 1433G; 1433Z; brain protein 14-3-3, beta isoform; EIEE56; epididymis luminal protein 4; epididymis secretory protein Li 1; epididymis secretory protein Li 3; epididymis secretory protein Li 93; GW128; HEL-S-1; HEL-S-3; HEL-S-93; HEL4; HS1; KCIP-1; MGC111427; MGC126532; MGC138156; phospholipase A2; POPCHAS; PPP1R170; Protein 1054; Protein AS1; Protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; protein phosphatase 1, regulatory subunit 170; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein eta; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alpha polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, delta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, zeta polypeptide; YWHA1; YWHAA; YWHAB; YWHAD; YWHAG; YWHAH; YWHAZ Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 28 Source/Isotype: Rabbit IgG