CST, 5269S, HMGA2 Antibody

Polyclonal Antibody for studying HMGA2. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, HMGA2 Antibody (CST #5269) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity HMGA2 Antibody detects endogenous levels of total HMGA2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino-terminus of human HMGA2 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background HMGA2 belongs to the family of high mobility group with AT-hook DNA binding domain. HMGA proteins are considered architectural transcription factors; they do not have direct transcriptional activation capacity, but instead regulate gene expression by changing DNA conformation through binding to AT-rich regions in the DNA and/or direct interaction with other transcription factors (1,2). HMGA2 is abundantly and ubiquitously expressed and plays a crucial role during embryonic development (3). HMGA2 promotes stem cell self-renewal and research studies have shown that decreased HMGA2 expression is associated with stem cell aging (2,4-6). Investigators have shown that expression levels of HMGA2 are very low in normal adult tissues, while either overexpression or rearrangement is associated with many types of cancer (7-10). Alternate Names BABL; high mobility group AT-hook 2; High mobility group AT-hook protein 2; High mobility group protein HMGI-C; high-mobility group (nonhistone chromosomal) protein isoform I-C; High-mobility group protein HMGI-C; HMGA2; HMGA2/KRT121P fusion; HMGI-C; HMGIC; LIPO; SRS5; STQTL9 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 18 SOURCE: Rabbit