CST, 52818S, FAP (F1A4G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FAP. Validated for WB,WB,IP,IHC,F,F. Available in 2 sizes. Highly specific and rigorously validated in-house, FAP (F1A4G) Rabbit Monoclonal Antibody (CST #52818) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:100 - 1:400 Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800 Flow Cytometry (Live): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 81192 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity FAP (F1A4G) Rabbit Monoclonal Antibody recognizes endogenous levels of total FAP protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to full-length human FAP protein. Background Fibroblast activation protein/seprase (FAP) is a cell surface serine integral membrane protease with roles in tissue remodeling during development and wound healing, as well as in cancer and fibrotic disease. FAP has both dipeptidyl peptidase and endopeptidase activities, and cleaves at N-terminal Xaa-Pro sequences. FAP is highly expressed in tumor stroma, and contributes to migration and invasion of tumor cells, as well as that of endothelial cells, into the extracellular matrix. FAP is a useful marker of tumorigenic stroma and of cancer-associated fibroblasts (CAFs) and fibrosis (1,2). Alternate Names 170 kDa melanoma membrane-bound gelatinase; Antiplasmin-cleaving enzyme FAP, soluble form; APCE; Dipeptidyl peptidase FAP; DKFZp686G13158; DPPIV; FAP; FAPA; FAPalpha; Fibroblast activation protein alpha; fibroblast activation protein, alpha; fibroblast activation protein, alpha subunit; Gelatine degradation protease FAP; Integral membrane serine protease; Post-proline cleaving enzyme; Prolyl endopeptidase FAP; SEPR; Seprase; Serine integral membrane protease; SIMP; Surface-expressed protease Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 90 Source/Isotype: Rabbit IgG