CST, 52827T, ULBP2 (E9G2P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ULBP2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, ULBP2 (E9G2P) Rabbit Monoclonal Antibody (CST #52827) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity ULBP2 (E9G2P) Rabbit Monoclonal Antibody recognizes endogenous levels of total ULBP2 protein. This antibody cross-reacts weakly with human ULBP4 and ULBP5 proteins. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant human ULBP2 protein. Background Natural killer group 2D (NKG2D) is an activating receptor expressed on NK and some T cells. It recognizes a variety of ligands that belong to the non-classical MHC class I family, including MICA, MICB, ULBP1-6 in human and Mult1, H60 (a, b, and c), and Rae-1 (α-ε) in mice. These ligand proteins are not expressed in most tissues under normal conditions, but are expressed in cells under stress, such as viral infection and malignant transformation. Engagement of NKG2D by these ligands activates cytolytic activity of NK and T cells against target cells, providing immunosurveillance. Some of these ligands are polymorphic. There are more than 100 different alleles for MICA, 40 for MICB, and around 20 for ULBPs (1-4). In addition, some of these ligands can be proteolytically cleaved from the cell surface to become soluble forms. The interaction of soluble ligands with NKG2D results in endocytosis and degradation of ligand-receptor complexes, therefore suppressing NKG2D-mediated immunosurveillance (5,6). However, recent data also demonstrated that soluble ligands can promote NK cell activation and tumor rejection (7,8). Alternate Names ALCAN-alpha; N2DL-2; N2DL2; NKG2D ligand 2; NKG2DL2; RAET1H; RAET1L; Retinoic acid early transcript 1H; retinoic acid early transcript 1L; UL16 binding protein 2; UL16-binding protein 2; ULBP2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 35 Source/Isotype: Rabbit IgG