CST, 52899SF, Cathepsin D (E7Z4L) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Cathepsin D mouse. Validated for WB,IHC,IF,IF. Highly specific and rigorously validated in-house, Cathepsin D (E7Z4L) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #52899) is ready to ship. Product Usage Information This product is the carrier free version of product #88239. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 88239 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Cathepsin D (E7Z4L) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of preprocathepsin D, procathepsin D and the heavy and light chain subunit of mature cathepsin D protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse cathepsin D protein. Background Cathepsin D is a ubiquitously expressed lysosomal aspartyl protease involved in the normal degradation of proteins (1). It is synthesized as an inactive 43 kDa preprocathepsin D that is cleaved and glycosylated to form a 46 kDa procathepsin D and then further cleaved to produce 28 kDa and 15 kDa subunits (heavy and light chains, respectively) (2). Cathepsin D may also be secreted into the cytosol during apoptosis and contribute to cleavage of substrates implicated in the apoptotic pathway (3). Numerous studies have suggested that cathepsin D plays a role in neuronal degradation and malignant transformation, particularly in breast cancer (4-9). Alternate Names Cat; CatD; Cathepsin D; CD; Ctsd; OTTMUSP00000020357 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 46, 43, 28 Source/Isotype: Rabbit IgG