CST, 52928T, Phospho-FGF Receptor 1 (Tyr653/654) (D4X3D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FGFR1 (Tyr653/Tyr654) phosphate. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-FGF Receptor 1 (Tyr653/654) (D4X3D) Rabbit Monoclonal Antibody (CST #52928) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 94995 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Phospho-FGF Receptor 1 (Tyr653/654) (D4X3D) Rabbit Monoclonal Antibody recognizes endogenous levels of FGFR1 protein when phosphorylated at Tyr653 and/or Tyr654. This antibody exhibits some cross-reactivity with FGF Receptors 2 and 4 when overexpressed exogenously. Based on sequence comparisons, cross-reactivity with FGF Receptor 3 is possible but has not been experimentally confirmed. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phospho-peptide corresponding to residues surrounding Tyr653 of human FGFR1 protein. Background Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through cell surface receptor tyrosine kinases. There are four members of the FGF receptor family: FGFR1 (flg), FGFR2 (bek, KGFR), FGFR3, and FGFR4. Each receptor contains an extracellular ligand-binding domain, a transmembrane domain, and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR1 can be phosphorylated: Tyr463, 583, 585, 653, 654, 730, and 766. Tyr653 and Tyr654 are important for catalytic activity of activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components, such as Crk and PLCγ (4,5). Alternate Names Basic fibroblast growth factor receptor 1; bFGF-R-1; BFGFR; CD331; CEK; ECCL; FGFBR; FGFR-1; FGFR1; FGFR1/PLAG1 fusion; Fibroblast growth factor receptor 1; FLG; FLJ99988; FLT-2; FLT2; Fms-like tyrosine kinase 2; fms-related tyrosine kinase 2; HBGFR; heparin-binding growth factor receptor; HH2; HRTFDS; hydroxyaryl-protein kinase; KAL2; N-SAM; OGD; Proto-oncogene c-Fgr; soluble FGFR1 variant 1; soluble FGFR1 variant 2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 120, 145 Source/Isotype: Rabbit IgG