CST, 5292S, BrdU (Bu20a) Mouse Monoclonal Antibody

Monoclonal Antibody for studying . Validated for Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, BrdU (Bu20a) Mouse Monoclonal Antibody (CST #5292) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:200 Immunofluorescence (Immunocytochemistry): 1:400 Flow Cytometry (Fixed/Permeabilized): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity BrdU (Bu20a) Mouse Monoclonal Antibody detects BrdU when incorporated into single stranded DNA. DNA must be denatured for the epitope to be exposed and recognized by the antibody. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with BrdU conjugated to BSA. Background Halogenated nucleotides such as the pyrimidine analog bromodeoxyuridine (BrdU) are useful for labeling nascent DNA in living cells and tissues. BrdU becomes incorporated into replicating DNA in place of thymidine and subsequent immunodetection of BrdU using specific monoclonal antibodies allows labeling of cells in S phase of the cell cycle. After pulse-labeling cells or tissues with bromodeoxyuridine, BrdU (Bu20a) Mouse mAb can be used to detect BrdU incorporated into single stranded DNA. Please see our detailed protocol for information regarding the labeling procedure and denaturation of double stranded DNA for various immunodetection applications (1-4). Specification REACTIVITY: All SENSITIVITY: Endogenous Source/Isotype: Mouse IgG1