CST, 52933S, HRI (F9Y7H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying HRI. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, HRI (F9Y7H) Rabbit Monoclonal Antibody (CST #52933) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity HRI (F9Y7H) Rabbit Monoclonal Antibody recognizes endogenous levels of total HRI protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala45 of human HRI protein. Background Heme-regulated inhibitor (HRI), a heme-regulated eukaryotic initiation factor 2α (eIF2α) kinase, is a cytosolic protein kinase that is highly expressed in erythroid cells (1,2). Upon stress conditions such as heme deficiency, oxidative stress, osmotic shock, mitochondrial dysfunction, and heat shock, HRI is a key activator of the integrated stress response (ISR) (3-7). Following activation, HRI is autophosphorylated, leading to eIF2α phosphorylation, which controls protein synthesis by inhibiting translational initiation (3-7). Alternate Names E2AK1; EIF2AK1; eukaryotic translation initiation factor 2 alpha kinase 1; Eukaryotic translation initiation factor 2-alpha kinase 1; HCR; heme regulated initiation factor 2 alpha kinase; heme sensitive initiation factor 2a kinase; Heme-controlled repressor; Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase; Heme-regulated inhibitor; heme-regulated initiation factor 2-alpha kinase; heme-regulated repressor; Hemin-sensitive initiation factor 2-alpha kinase; hHRI; HRI; KIAA1369; LEMSPAD Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 82 Source/Isotype: Rabbit IgG