CST, 5294S, Phospho-CTDSPL2 (Ser104) Antibody

Polyclonal Antibody for studying CTDSPL2 (Ser104) phosphate. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Phospho-CTDSPL2 (Ser104) Antibody (CST #5294) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-CTDSPL2 (Ser104) Antibody detects endogenous levels of CTDSPL2 only when phosphorylated at Ser104. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser104 of human CTDSPL2 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background CTD small phosphatase-like protein 2 (CTDSPL2, HSPC129) is a putative RNA-polymerase II carboxy-terminal domain (CTD) phosphatase (1) that belongs to a small subfamily of CTD phosphatases (2). The CTD of RNA polymerase II contains multiple Y-S-P-T-S-P-S repeats that are phosphorylated during the transcription cycle (3,4). In general, CTD phosphatases regulate the reversible CTD phosphorylation state of RNA-polymerase II at several stages of RNA synthesis and during post-transcriptional modification (4-6). CTDSPL2 has several structural and functional similarities to other CTD phosphatases, including FCP1, SCP1, DULLARD, and UBLCP1 (1,2). Phosphorylation of CTDSPL2 at Ser104 was identified at Cell Signaling Technology (CST) using PhosphoScan , CST's LC-MS/MS platform for phosphorylation site discovery (7). The site was independently found in select carcinoma cell lines and in tumors (8). Alternate Names CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase like 2; CTD small phosphatase like 2; CTD small phosphatase-like protein 2; CTDSP-like 2; CTDSPL2; CTSL2; FLJ10523; HSPC058; HSPC129; SCP4 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 61 SOURCE: Rabbit