CST, 5295S, ADH1 Antibody

Polyclonal Antibody for studying ADH1B. Validated for Western Blotting. Highly specific and rigorously validated in-house, ADH1 Antibody (CST #5295) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity ADH1 Antibody detects endogenous levels of total ADH1 protein. The antigen is 100% conserved between human ADH1A, ADH1B and ADH1C proteins. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val74 of human ADH1B protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Human alcohol dehydrogenase ( ) genes are grouped into five classes, with three distinct class I genes ( , and ) and , , and belonging to classes II, III, IV, and V, respectively. ADH is a zinc-containing, dimeric enzyme that catalyzes the conversion of cytosolic alcohol to acetaldehyde in the liver with the coenzyme NAD (1). ADH1A is monomorphic and is the predominant fetal and neonatal liver ADH enzyme. In contrast, polymorphic ADH1B and ADH1C enzymes are predominant in adult livers (2). Polymorphisms in the human class I genes result in functionally variable ADH enzymes; evidence suggests that specific variants may provide protection from the risk of alcoholism (3). Alternate Names ADH, beta subunit; ADH1B; ADH2; Alcohol dehydrogenase 1B; alcohol dehydrogenase 1B (class I), beta polypeptide; alcohol dehydrogenase 2 (class I), beta polypeptide; Alcohol dehydrogenase subunit beta; aldehyde reductase; All-trans-retinol dehydrogenase [NAD(+)] ADH1B; DKFZp686C06125; epididymis secretory protein Li 117; HEL-S-117 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 40 SOURCE: Rabbit