CST, 52975S, Perilipin-1 (D1D8) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate)

Monoclonal Antibody for studying perilipin. Validated for Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Perilipin-1 (D1D8) Rabbit Monoclonal Antibody (Alexa Fluor^® 647 Conjugate) (CST #52975) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:50 - 1:200 Immunofluorescence (Immunocytochemistry): 1:50 - 1:200 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Perilipin-1 (D1D8) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues around Ile419 of human perilipin-1 protein. Background Triacylglycerol is stored in lipid droplets as a primary energy reserve. Perilipin is localized at the periphery of lipid droplets and serves as a protective coating against lipases (1-3). Evidence suggests that PKA regulates lipolysis by phosphorylating perilipin (1,2,4,5). Phosphorylation of perilipin results in the conformational change that exposes lipid droplets to endogenous lipases, such as hormone-sensitive lipases (2). Hence, perilipin plays a pivotal role in lipid storage (2,5). Alternate Names FPLD4; Lipid droplet-associated protein; PERI; perilipin 1; Perilipin-1; PLIN; PLIN1 Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG