CST, 52976SF, Integrin beta6 (E4M9P) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Integrin Beta6. Validated for WB,IHC,IHC,IF. Highly specific and rigorously validated in-house, Integrin beta6 (E4M9P) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #52976) is ready to ship. Product Usage Information This product is the carrier free version of product #95153. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 95153 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Integrin beta6 (E4M9P) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total integrin β6 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human integrin β6 protein. Background Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and eight β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling) but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1,2). The avβ6 subunit was identified in epithelial cells and interacts with the fibronectin receptor integrin α5 (3,4). It is expressed during development, but expression becomes repressed in adulthood (5). It is involved in wound healing, fibrosis, pulmonary fibrosis in particular, and tumorigenesis (6-8). When the dimer is unregulated in the membrane in response to injury, the integrin β6 subunit binds directly to an RGD motif present in fibronectin and TGF-β, both present in the extracellular matrix. Integrin β6 cleaves and binds the LAP fragment of latent TGF-β, thereby releasing active TGF-β into the ECM to activate myofibroblasts, which consequently mediate ECM remodeling (6-8). Alternate Names AI1H; Integrin beta-6; integrin subunit beta 6; integrin, beta 6; ITB6; ITGB6 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 105 Source/Isotype: Rabbit IgG