CST, 5297L, Synapsin-1 (D12G5) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Synapsin-1. Validated for WB,WB,IP,IHC,IF. Available in 3 sizes. Highly specific and rigorously validated in-house, Synapsin-1 (D12G5) Rabbit Monoclonal Antibody (CST #5297) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:400 - 1:1600 Immunofluorescence (Frozen): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product # 53059 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen) Specificity / Sensitivity Synapsin-1 (D12G5) Rabbit Monoclonal Antibody detects endogenous levels of total synapsin protein. The antigen is 100% conserved between human synapsin-1a and synapsin-1b. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln483 of human synapsin-1 protein. Background Synapsins, a group of at least five related members (synapsins Ia, Ib, IIa, IIb, and IIIa), are abundant brain proteins essential for regulating neurotransmitter release (1,2). All synapsins contain a short amino-terminal domain that is highly conserved and phosphorylated by PKA or CaM kinase I (1). Phosphorylation of the synapsin amino-terminal domain at Ser9 inhibits its binding to phospholipids and dissociates synapsins from synaptic vesicles (2). Alternate Names Brain protein 4.1; MRX50; SYN1; SYN1a; SYN1b; Synapsin; Synapsin I; synapsin Ib; Synapsin-1; SYNI Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 77 Source/Isotype: Rabbit IgG