CST, 5305S, GAD1 Antibody

Polyclonal Antibody for studying GAD1. Validated for Western Blotting. Highly specific and rigorously validated in-house, GAD1 Antibody (CST #5305) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity GAD1 Antibody detects endogenous levels of total GAD1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro19 of human GAD1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The enzyme glutamate decarboxylase (GAD) is responsible for the synthesis of the essential neurotransmitter gamma-aminobutyric acid (GABA) from L-glutamic acid (1). GAD1 (GAD67) and GAD2 (GAD65) are expressed in nervous and endocrine systems (2) and are thought to be involved in synaptic transmission (3) and insulin secretion (4), respectively. Autoantibodies against GAD2 may serve as markers for type I diabetes (5). Many individuals suffering from an adult onset disorder known as Stiff Person Syndrome (SPS) also express autoantibodies to GAD2 (6). Mutations in the gene can cause autosomal recessive spastic cerebral palsy, possibly attributable to altered glutamate/GABA ratios (7). Alternate Names 67 kDa glutamic acid decarboxylase; CPSQ1; DCE1; FLJ45882; GAD; GAD-67; GAD1; GAD67; Glutamate decarboxylase 1; glutamate decarboxylase 1 (brain, 67kDa); Glutamate decarboxylase 67 kDa isoform; SCP Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 67 SOURCE: Rabbit