CST, 53069SF, EED (E4L6E) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying EED. Validated for WB,IHC,IF,F. Highly specific and rigorously validated in-house, EED (E4L6E) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #53069) is ready to ship. Product Usage Information This product is the carrier free version of product #85322. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 85322 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity EED (E4L6E) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total EED protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human EED protein. Background The polycomb group (PcG) proteins contribute to the maintenance of cell identity, stem cell self-renewal, cell cycle regulation and oncogenesis by maintaining the silenced state of genes that promote cell lineage Specification, cell death and cell-cycle arrest (1-4). PcG proteins exist in two complexes that cooperate to maintain long-term gene silencing through epigenetic chromatin modifications. The first complex, EED-EZH2, is recruited to genes by DNA-binding transcription factors and methylates histone H3 on Lys27. Methylation of Lys27 facilitates the recruitment of the second complex, PRC1, which ubiquitinylates histone H2A on Lys119 (5). Embryonic ectoderm development protein (EED) is a component of the PRC2 complex, which together with Ezh2 and SUZ12 is absolutely required for histone methyl-transferase activity (6). EED, SUZ12 and EZH2 are overexpressed in various types of cancer, including tumors of the colon, breast, prostate and ovary (7-9). Alternate Names COGIS; EED; embryonic ectoderm development; Embryonic ectoderm development protein; hEED; Polycomb protein EED; WAIT-1; WAIT1; WD protein associating with integrin cytoplasmic tails 1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 50-70 Source/Isotype: Rabbit IgG