CST, 53096S, IFITM3 (F6U6U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IFITM3 mouse. Validated for WB,IP,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, IFITM3 (F6U6U) Rabbit Monoclonal Antibody (CST #53096) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:100 - 1:400 Immunofluorescence (Frozen): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen) Specificity / Sensitivity IFITM3 (F6U6U) Rabbit mAb recognizes endogenous levels of total IFITM3 protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr20 of mouse IFITM3 protein. Background Interferon-induced transmembrane protein (IFITM) family members are composed of short amino- and carboxy-termini, two transmembrane domains, and a cytoplasmic domain (1). There are four family members in humans: IFITM1, IFITM2, IFITM3, and IFITM5 (2,3). Mice have two additional family members, IFITM6 and IFITM7 (2,3). Basal expression of IFITM proteins is observed in some cells and expression can also be induced by type I and type II interferons (4-6). The primary function of IFITM family proteins appears to be viral restriction, as IFITM proteins inhibit cytosolic entry of viruses by preventing fusion of viral and host membranes (7,8). The mechanism by which IFITM proteins inhibit fusion is unclear. Although IFITM proteins are present on both the plasma membrane and intracellular membranes, they most effectively restrict viral fusion in late endosomes and lysosomes (8,9). In addition, different family members exhibit specific viral preferences (9). For example, IFITM3 is most effective at restricting influenza A infection, while IFITM1 is more successful in controlling filoviruses and SARS (9,10). Alternate Names 1110004C05Rik; Cd225; Cdw217; Dispanin subfamily A member 2b; DSPA2b; Fgls; Fragilis; Fragilis protein; ifitm-like protein 1; Ifitm3; IFM3; Interferon induced transmembrane protein 3; Interferon-induced transmembrane protein 3; Interferon-inducible protein 15; IP15; MCG22590; mil; mil-1; Mouse ifitm-like protein 1 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 13-15 Source/Isotype: Rabbit IgG