CST, 5311S, PTP1B Antibody

Polyclonal Antibody for studying PTP1B. Validated for Western Blotting. Highly specific and rigorously validated in-house, PTP1B Antibody (CST #5311) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PTP1B Antibody detects endogenous levels of total PTP1B protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PTP1B protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Protein-tyrosine phosphatase 1B (PTP1B) is the original member of the protein tyrosine phosphatase (PTP) family of proteins (1). PTP1B is highly expressed in insulin-responsive tissues and negatively regulates insulin, as well as integrin, through dephosphorylation of phosphotyrosine residues (2-4). PTP1B knockout mice show resistance to dietary weight gain and enhanced insulin sensitivity, suggesting a possible role in treatment of obesity as well as type 2 diabetes (5). Alternate Names protein tyrosine phosphatase 1B; protein tyrosine phosphatase non-receptor type 1; protein tyrosine phosphatase, non-receptor type 1; protein tyrosine phosphatase, placental; Protein-tyrosine phosphatase 1B; PTN1; PTP-1B; PTP1B; PTPN1; Tyrosine-protein phosphatase non-receptor type 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 50 SOURCE: Rabbit