CST, 5313S, RBPSUH (D10A4) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RBPSUH. Validated for Western Blotting,Immunohistochemistry (Paraffin),Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, RBPSUH (D10A4) Rabbit Monoclonal Antibody (CST #5313) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:2600 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 26978 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Chromatin IP Specificity / Sensitivity RBPSUH (D10A4) Rabbit Monoclonal Antibody recognizes endogenous levels of total RBPSUH protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln110 of human RBPSUH protein. Background RBPSUH ( ecombining inding rotein, ppressor of airless), also termed RBP-J or CSL, is the DNA-binding component of the transcription complex regulated by canonical Notch signaling. In the absence of Notch activation, RBPSUH suppresses target gene expression through interactions with a co-repressor complex containing histone deacetylase. Upon activation of Notch receptors, the Notch intracellular domain (NICD) translocates to the nucleus and binds to RBPSUH. This displaces the co-repressor complex and replaces it with a transcription activation complex that includes Mastermind-like (MAML) proteins and histone acetylase p300, leading to transcriptional activation of Notch target genes (1-3). RBPSUH is also the DNA-binding partner for Epstein-Barr virus (EBV) nuclear antigen 2 (EBNA2), a protein critical for latent viral transcription and immortalization of EBV-infected B cells (4,5). Alternate Names AOS3; CBF-1; CBF1; csl; H-2K binding factor-2; IGKJRB; IGKJRB1; immunoglobulin kappa J region recombination signal binding protein 1; J kappa-recombination signal-binding protein; KBF2; MGC61669; RBP-J; RBP-J kappa; RBP-JK; RBPJ; RBPJK; RBPSUH; recombination signal binding protein for immunoglobulin kappa J region; Recombining binding protein suppressor of hairless; Renal carcinoma antigen NY-REN-30; SUH; suppressor of hairless homolog Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 61 Source/Isotype: Rabbit IgG