CST, 53142SF, Merlin/Ezrin/Radixin/Moesin (D1P8I) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Merlin. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Merlin/Ezrin/Radixin/Moesin (D1P8I) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #53142) is ready to ship. Product Usage Information This product is the carrier free version of product #23292. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 23292 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Merlin/Ezrin/Radixin/Moesin (D1P8I) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total merlin, ezrin, radixin, and moesin protein. This antibody immunoprecipitates total merlin protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human merlin protein. Background Neurofibromatosis 2 (NF2) is an autosomal dominant, inherited disorder characterized by the occurrence of vestibular schwannomas, meningiomas, and other nervous system tumors. Both the familial tumors of NF2 and equivalent sporadic tumors found in the general population are caused by inactivation of the tumor suppressor gene. Merlin (moesin, ezrin, and radixin-like protein) is the gene product, displaying striking similarity to ezrin, radixin, and moesin (ERM) proteins. Regulation of merlin (also called schwannomin) and ERM proteins involves intramolecular and intermolecular head-to-tail associations between family members (1). Merlin and ERM proteins act as linkers between the plasma membrane and the cytoskeleton, affecting cell morphology, polarity, and signal transduction (2). Merlin is phosphorylated by the Rac/Cdc42 effector p21-activated kinase (PAK) at Ser518, negatively regulating Rac (3,4). Alternate Names ACN; BANF; MERL; Merlin; moesin-ezrin-radixin like; Moesin-ezrin-radixin-like protein; moesin-ezrin-radizin-like protein; neurofibromin 2; neurofibromin 2 (bilateral acoustic neuroma); neurofibromin 2 (merlin); Neurofibromin-2; NF2; SCH; Schwannomerlin; Schwannomin Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 70 Merlin. 75 Moesin. 80 Ezrin & Radixin. Source/Isotype: Rabbit IgG