CST, 53151T, Fc-gammaRIIB (E4D2F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FcgR2b. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Fc-gammaRIIB (E4D2F) Rabbit Monoclonal Antibody (CST #53151) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 57415 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity Fc-gammaRIIB (E4D2F) Rabbit Monoclonal Antibody recognizes endogenous levels of total FcγRIIB protein. This antibody does not cross-react with FcγRIIA protein. Goat serum should not be used in the blocking reagent or diluent for immunohistochemistry. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu303 of human FcγRIIB protein. Background FcγRIIB (CD32B) is a low affinity, IgG Fc-binding receptor expressed on B cells, monocytes, macrophages, and dendritic cells (DCs) (1-3). It is the inhibitory Fc receptor and signals through an immunoreceptor tyrosine-based inhibitory motif (ITIM) within its carboxy-terminal cytoplasmic tail (2). Binding of immune complexes to FcγRIIB results in tyrosine phosphorylation of the ITIM motif at Tyr292 and recruitment of the phosphatase SHIP, which mediates inhibitory effects on immune cell activation (2,4). In this way, FcγRIIB suppresses the effects of activating Fc-binding receptors (3). For example, mice deficient for FcγRIIB have greater T cell and DC responses following injection of immune complexes (5,6). In addition, FcγRIIB plays a role in B cell affinity maturation (7). Signaling through FcγRIIB in the absence of signaling through the B cell receptor (BCR) is proapoptotic, while signaling through FcγRIIB and the BCR simultaneously attenuates the apoptotic signal and results in selection of B cells with higher antigen affinity (7). Alternate Names CD32; CD32B; CDw32; Fc fragment of IgG receptor IIb; Fc fragment of IgG, low affinity II, receptor for (CD32); Fc fragment of IgG, low affinity IIb, receptor (CD32); Fc fragment of IgG, low affinity IIb, receptor for (CD32); Fc gamma receptor IIb; Fc gamma RIIb; Fc-gamma RII-b; Fc-gamma RII-c; Fc-gamma-RIIb; Fc-gamma-RIIc; FCG2; FCG2B; FCGR2; FCGR2B; FCGR2C; FcRII-b; FcRII-c; IGFR2; IgG Fc receptor II-b; IgG Fc receptor II-c; Low affinity immunoglobulin gamma Fc region receptor II-b; Low affinity immunoglobulin gamma Fc region receptor II-c Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 40-50 Source/Isotype: Rabbit IgG