CST, 5318S, AIF (D39D2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PDCD8. Validated for WB,IP,IHC,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, AIF (D39D2) Rabbit Monoclonal Antibody (CST #5318) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:50 - 1:200 Immunofluorescence (Frozen): 1:200 - 1:400 Immunofluorescence (Immunocytochemistry): 1:200 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 62147 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity AIF (D39D2) Rabbit Monoclonal Antibody detects endogenous levels of total AIF protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody was produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala520 of human AIF protein. Background Apoptosis-inducing factor (AIF, PDCD8) is a ubiquitously expressed flavoprotein that plays a critical role in caspase-independent apoptosis (reviewed in 1,2). AIF is normally localized to the mitochondrial intermembrane space and released in response to apoptotic stimuli (3). Treatment of isolated nuclei with recombinant AIF leads to early apoptotic events, such as chromatin condensation and large-scale DNA fragmentation (3). Studies of AIF knockout mice have shown that the apoptotic activity of AIF is cell type and stimuli-dependent. Also noted was that AIF was required for embryoid body cavitation, representing the first wave of programmed cell death during embryonic morphogenesis (4). Structural analysis of AIF revealed two important regions, the first having oxidoreductase activity and the second being a potential DNA binding domain (3,5). While AIF is redox-active and can behave as an NADH oxidase, this activity is not required for inducing apoptosis (6). Instead, recent studies suggest that AIF has dual functions, a pro-apoptotic activity in the nucleus via its DNA binding and an anti-apoptotic activity via the scavenging of free radicals through its oxidoreductase activity (2,7). Alternate Names AIF; AIFM1; apoptosis inducing factor mitochondria associated 1; Apoptosis-inducing factor 1, mitochondrial; apoptosis-inducing factor, mitochondrion-associated, 1; auditory neuropathy, X-linked recessive 1; AUNX1; CMT2D; CMTX4; COWCK; COXPD6; DFNX5; MGC111425; NADMR; NAMSD; PDCD8; programmed cell death 8 (apoptosis-inducing factor); Programmed cell death protein 8; SEMDHL; striatal apoptosis-inducing factor; testicular secretory protein Li 4 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 67 Source/Isotype: Rabbit IgG