CST, 53229T, USP18 (E9K4X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying USP18 mouse. Validated for WB,WB,IP,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, USP18 (E9K4X) Rabbit Monoclonal Antibody (CST #53229) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:800 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity USP18 (E9K4X) Rabbit Monoclonal Antibody recognizes endogenous levels of total USP18 protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly50 of mouse USP18 protein. Background Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action. Five DUB subfamilies are recognized, including the USP, UCH, OTU, MJD, and JAMM enzymes (1,2). USP18 (also known as UBP43) is a deubiquitinase best known for catalyzing the removal of ISG15, an interferon-regulated ubiquitin-like protein, from conjugated proteins (3). Removal of ISG15 from target proteins by the USP18 peptidase maintains the critical cellular balance of ISG15-conjugated proteins important for normal development and brain function (4,5). Following induction by IFN or LPS (6), USP18 binds the INF receptor subunit IFNAR2 and inhibits signal transduction through the Jak/Stat pathway (7). USP18 regulation of IFN signaling inhibits IFN-mediated apoptosis and does not necessarily rely on USP18 peptidase activity (8). As the therapeutic use of recombinant IFN can lead to refractory IFN signaling and a less effective response, the combination of IFN treatment and regulation of USP18 expression may produce a more positive outcome (9). Alternate Names 1110058H21Rik; 43 kDa ISG15-specific protease; AW047653; ISG15-specific-processing protease; ubiquitin specific peptidase 18; ubiquitin specific protease 15; ubiquitin specific protease 18; Ubl carboxyl-terminal hydrolase 18; Ubl thioesterase 18; ubl thiolesterase 18; Ubp15; UBP18; UBP4; Ubp43; Usp18 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 40 Source/Isotype: Rabbit IgG