CST, 5324S, eIF2 alpha (D7D3) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying EIF2S1/eIF2-alpha. Validated for WB,WB,IP,IHC,eCLIP. Available in 3 sizes. Highly specific and rigorously validated in-house, eIF2 alpha (D7D3) Rabbit Monoclonal Antibody (CST #5324) is ready to ship. Product Usage Information For more information about the RBP-eCLIP service please visit Eclipsebio . Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:800 - 1:3200 eCLIP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 62928 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity eIF2 alpha (D7D3) Rabbit Monoclonal Antibody detects endogenous levels of total eIF2α protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a purified recombinant protein fragment representing sequence in the central region of human eIF2α. Background Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. eIF2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B (1,2). Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the α subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51 (5,6). Alternate Names EIF-2; eIF-2-alpha; eIF-2A; eIF-2alpha; EIF2; eIF2-alpha; EIF2A; EIF2S1; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa; IF2A Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 38 Source/Isotype: Rabbit IgG