CST, 53303SF, ILK1 (4G9) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying ILK1. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, ILK1 (4G9) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #53303) is ready to ship. Product Usage Information This product is the carrier free version of product #3856. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 3856 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity ILK1 (4G9) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total ILK1 protein. This antibody may cross-react with ILK2. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey, Zebrafish, Bovine, Pig Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human ILK1. Background Integrin-linked kinases (ILKs) couple integrins and growth factors to downstream pathways involved in cell survival, cell cycle control, cell-cell adhesion and cell motility (1). ILK functions as a scaffold bridging the extracellular matrix (ECM) and growth factor receptors to the actin cytoskeleton through interactions with integrin, PINCH (which links ILK to the RTKs via Nck2), CH-ILKBP and affixin (1). ILK phosphorylates Akt at Ser473, GSK-3 on Ser9, myosin light chain 2 (MLC2) on Ser18/Thr19, as well as affixin (2-5). These phosphorylation events are key regulatory steps in modulating the activities of the targets. ILK activity is stimulated by PI3 kinase and negatively regulated by the tumor suppressor PTEN and a PP2C protein phosphatase, ILKAP (1,3,6). It has been suggested that the conserved Ser343 residue in the activation loop plays a key role in the activation of ILK1 (2). Alternate Names 59 kDa serine/threonine-protein kinase; DKFZp686F1765; epididymis secretory protein Li 28; HEL-S-28; ILK; ILK-1; ILK-2; ILK1; ILK2; integrin linked kinase; integrin-linked kinase; integrin-linked kinase-2; Integrin-linked protein kinase; P59; p59ILK Specification REACTIVITY: H M R Hm Mk Z B Pg SENSITIVITY: Endogenous MW (kDa): 51 Source/Isotype: Rabbit IgG