CST, 53370S, Xanthine Dehydrogenase/Oxidase Antibody

Polyclonal Antibody for studying XDH. Validated for Western Blotting. Highly specific and rigorously validated in-house, Xanthine Dehydrogenase/Oxidase Antibody (CST #53370) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Xanthine Dehydrogenase/Oxidase Antibody recognizes endogenous levels of total xanthine dehydrogenase/oxidase protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu560 of human xanthine dehydrogenase/oxidase protein. Antibodies are purified by peptide affinity chromatography. Background Xanthine dehydrogenase/oxidase is a rate-limiting enzyme in purine degradation. It catalyzes the oxidation of hypoxanthine to xanthine and the oxidation of xanthine to uric acid. The circadian rhythm of the expression of this enzyme and its enzymatic activity lead to circadian oscillation of uric acid concentrations in the liver and blood (1). In macrophages, the histone methyltransferase Setdb2 mediates the elevated expression of xanthine dehydrogenase/oxidase, and consequently, the increased production of uric acid. Pharmacological targeting of the uric acid pathway of purine degradation improves wound healing (2). In addition, research studies show that the xanthine oxidase activity of this enzyme contributes to the reactive oxygen species generation in macrophages and is essential in innate inflammatory signaling (3). Alternate Names XAN1; xanthene dehydrogenase; Xanthine dehydrogenase; Xanthine dehydrogenase/oxidase; Xanthine oxidase; Xanthine oxidoreductase; XD; XDH; XDHA; XO; XOR Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 146 SOURCE: Rabbit