CST, 53385T, WDR59 (D4Z7A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying WDR59. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, WDR59 (D4Z7A) Rabbit Monoclonal Antibody (CST #53385) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity WDR59 (D4Z7A) Rabbit Monoclonal Antibody recognizes endogenous levels of total WDR59 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His356 of human WDR59 protein. Background The mTORC1 kinase complex plays a critical role in cell growth regulation (1, 2). mTORC1 activity is modulated by cellular environmental factors (e.g., energy levels, growth factors, amino acids) (3, 4), which are mediated through a protein complex known as GATOR (GAP Activity Towards Rags) (5). GATOR is comprised of two protein subcomplexes (GATOR1 and GATOR2), that function in opposing fashion to regulate mTORC1 activity (5). WDR59 was identified as a component of the GATOR2 subcomplex (also containing Mios, WDR24, Seh1L and Sec13), that functions to positively regulate mTORC1 activity (5). Conversely, the GATOR1 subcomplex (containing DEPDC5, Nprl2 and Nprl3), negatively regulates mTORC1 activity, through activation of RagA and RagB GTPases (5). Alternate Names CDW12; CDw12 antigen; FLJ12270; FP977; GATOR complex protein WDR59; GATOR2 complex protein WDR59; KIAA1923; MGC11230; p90-120; WD repeat domain 59; WD repeat-containing protein 59; WDR59 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 110 Source/Isotype: Rabbit IgG