CST, 53391SF, CD33 (E6V7H) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying CD33. Validated for Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, CD33 (E6V7H) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #53391) is ready to ship. Product Usage Information This product is the carrier free version of product #60049. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 60049 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity CD33 (E6V7H) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total CD33 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human CD33 protein. Background CD33, a type I transmembrane protein, is a sialic acid-binding Ig-like lectin 3 (Siglec-3) of the Ig superfamily, and human CD33 binds preferentially to alpha-2, 6-linked sialic acid. Upon binding to its ligands, CD33 transduces inhibitory signaling through the immunoreceptor tyrosine-based inhibitory motif (ITIM) in its intracellular domain, inhibiting cellular functions such as phagocytosis. In addition, CD33 is also involved in other processes, such as adhesion (1-3). Due to its exclusive expression on hematopoietic cells, particularly the myeloid lineage and their progenitors, CD33 has been actively pursued as a therapeutic target against acute myeloid leukemia (AML) (4,5). CD33 may also be involved in Alzheimer's Disease (AD) (6-8). Alternate Names CD33; CD33 antigen (gp67); CD33 molecule; CD33 molecule transcript; FLJ00391; gp67; Myeloid cell surface antigen CD33; p67; sialic acid binding Ig-like lectin 3; Sialic acid-binding Ig-like lectin 3; Siglec-3; SIGLEC3 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 70-80 Source/Isotype: Rabbit IgG