CST, 53452S, Cell Proliferation Tracer Kit, (Fluorometric, Blue 520)

Assay Kit for studying in the research area. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Background Due to their inherent metabolic stability once inside a cell, fluorescent proliferation dyes partition in an equal manner between daughter cells during the M phase of the cell cycle. This allows the principle of dye dilution to be leveraged as a means to trace multiple rounds of cell proliferation using flow cytometry. Added benefits of proliferation dyes are that they are non-radioactive and do not require cells to be actively synthesizing DNA for efficient uptake (1-3). Alternate Names Cell proliferation; Dye dilution