CST, 53460T, CD2 (D5L3B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, CD2 (D5L3B) Rabbit Monoclonal Antibody (CST #53460) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CD2 (D5L3B) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala36 of human CD2 protein. Background CD2 is a transmembrane glycoprotein expressed early in thymocyte development and present on most circulating T cells (1,2). CD2 plays a role in T cell adhesion through binding to its ligand CD58 (LFA-3) (3). Stimulation of CD2 also leads to T cell activation and proliferation (2). T cells from mice deficient in both CD2 and CD28 have severe defects in T cell activation and function, while T cells deficient in either CD2 or CD28 are still capable of mounting a response, suggesting that CD2 and CD28 may have overlapping functions and may be able to compensate for each other (4). In addition, engagement of CD2 and CD58 was recently demonstrated to be the primary costimulatory signal in T cells that lack CD28 (5). CD2 expression also distinguishes a subset of plasmacytoid dendritic cells found in tumors and tonsils that express lysozyme, higher levels of IL-12 p40, and higher levels of CD80 (6). Alternate Names CD2; CD2 antigen (p50), sheep red blood cell receptor; CD2 molecule; Erythrocyte receptor; FLJ46032; LFA-2; LFA-3 receptor; lymphocyte-function antigen-2; Rosette receptor; SRBC; T-cell surface antigen CD2; T-cell surface antigen T11/Leu-5; T11 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 48-65 Source/Isotype: Rabbit IgG