CST, 5347S, LAT1 Antibody

Polyclonal Antibody for studying SLC7A5. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, LAT1 Antibody (CST #5347) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity LAT1 Antibody detects endogenous levels of total LAT1 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the amino terminus of human LAT1 protein. Antibodies were purified by protein A and peptide affinity chromatography. Background L-type amino acid transporter 1 (LAT1), also known as solute carrier family 7 member 5 (SLC7A5), is a high-affinity neutral transporter of larger amino acids. It facilitates the cellular amino acid uptake in a sodium independent manner (1-2) and selectively transports D-and L-isomers of small neutral amino acids (3). LAT1 also regulates amino acid exchange in conjunction with solute carrier family 1 member 5 (SLC1A5) (2,4-6). Transport of thyroid hormones across the placenta is established via LAT1 during normal fetal development (7). LAT1 promotes neuronal cell proliferation by regulating the transport of amino acids across the blood brain barrier (8). LAT1 is upregulated in various cancer types, including breast cancer, lung cancer, prostate cancer, and gliomas (9,10). High expression of LAT1 is detected in non-small cell lung cancer with lymph node metastases (9,11,12). Increased LAT1 expression is a novel biomarker of high grade malignancy in prostate cancers (12). Inhibition of LAT1 suppresses tumor cell growth in several tumor types (10,13). Alternate Names 4F2 LC; 4F2 light chain; 4F2LC; CD98; CD98 light chain; CD98LC; D16S469E; E16; hLAT1; Integral membrane protein E16; L-type amino acid transporter 1; large neutral amino acids transporter 1; Large neutral amino acids transporter small subunit 1; LAT1; MPE16; SLC7A5; sodium-independent neutral amino acid transporter LAT1; solute carrier family 7 (amino acid transporter light chain, L system), member 5; solute carrier family 7 (cationic amino acid transporter, y+ system), member 5; Solute carrier family 7 member 5; y+ system cationic amino acid transporter Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 39 SOURCE: Rabbit