CST, 53507T, Vitronectin (F8D3L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying VTN. Validated for Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Vitronectin (F8D3L) Rabbit Monoclonal Antibody (CST #53507) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunohistochemistry (Paraffin) Specificity / Sensitivity Vitronectin (F8D3L) Rabbit Monoclonal Antibody recognizes endogenous levels of total vitronectin protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu133 of human vitronectin protein. Background Vitronectin (VTN) is a multidomain adhesive glycoprotein synthesized mostly in the liver and secreted to blood plasma and extracellular matrix (ECM) (1,2). The protein has an N-terminal SMB fragment mediating binding to PAI-1 and UPAR, followed by an RGD region mediating its interaction with integrins, and C-terminal hemopexin-like domains mediating its interaction with various ECM components such as collagens, tenascins, and plasminogen (2,3). VTN has been implicated as a regulator of many diverse physiological processes, including coagulation, complement-dependent immune response, cell attachment and spreading, angiogenesis, hemostasis, and vascular remodeling (4-6). Increased VTN expression promotes cancer cell migration, adhesion, and metastasis in neuroblastoma, ovarian cancer, and breast cancer cells (7-9). Alternate Names complement S-protein; epibolin; S-protein; serum spreading factor; Serum-spreading factor; somatomedin B; Somatomedin-B; V75; Vitronectin; vitronectin (serum spreading factor, somatomedin B, complement S-protein); Vitronectin V10 subunit; Vitronectin V65 subunit; VN; VNT; VTN; VTNC Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG