CST, 53541S, SerpinB9 (E9X9Z) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate)

Monoclonal Antibody for studying SERPINB9. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, SerpinB9 (E9X9Z) Rabbit Monoclonal Antibody (Alexa Fluor^® 488 Conjugate) (CST #53541) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity SerpinB9 (E9X9Z) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with human SERPINB9 recombinant protein and reacts with an epitope surrounding Met280. Background SERPINB9 (Sb9, PI-9) is a member of the serpin superfamily of proteins which bind to and inactivate serine proteinases (1,2). SERPINB9 is present in human CD4 T cells, CD8 T cells, NK cells, B cells, and myeloid cells, and is a physiological inhibitor of Granzyme B (GrB) (1,3,4). Granzyme B triggers apoptosis when released by cytotoxic T lymphocytes (1-4). SERPINB9 may function to protect effector and bystander cells from Granzyme B during an immune response (4). In mice, SPI6 is thought to be the functional homolog of SERPINB9 due to its inhibition of murine Granzyme B (2,5). SERPINB9 was recently identified as a potential immunotherapy target, due to its role in tumor cell survival through evasion of apoptosis (6). Alternate Names CAP-3; CAP3; Cytoplasmic antiproteinase 3; Peptidase inhibitor 9; PI-9; PI9; protease inhibitor 9 (ovalbumin type); serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 9; Serpin B9; serpin family B member 9; serpin peptidase inhibitor, clade B (ovalbumin), member 9; serpin peptidase inhibitor, clade B, member 9; SERPINB9; SPB9; testicular tissue protein Li 180 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG