CST, 53600S, SHOC2 (D7N1A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SHOC2. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, SHOC2 (D7N1A) Rabbit Monoclonal Antibody (CST #53600) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 55106 . Protocol Available protocols: Western Blotting Specificity / Sensitivity SHOC2 (D7N1A) Rabbit Monoclonal Antibody recognizes endogenous levels of total SHOC2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu520 of human SHOC2 protein. Background SHOC2 is a scaffolding protein that harbors multiple leucine-rich repeats in tandem and is an upstream positive regulator of growth factor-dependent MAPK/ERK signaling. Research studies have demonstrated that SHOC2 forms a complex with the catalytic subunit of the PP1 phosphatase and M-Ras, and this complex drives activation of Raf-ERK signaling in response to mitogenic growth factors (1). SHOC2 has also been shown to cross-talk with and activate the PI3K/Akt signaling axis through its interaction with the p110α catalytic subunit of PI3K (2). As a positive regulator of ERK and PI3K/Akt signaling cascades, SHOC2 has been implicated in the regulation of several oncogenic cellular processes such as cell motility, invasion, and metastasis (2). A mutation in that introduces an N-terminal myristoylation site, promotes aberrant membrane targeting of SHOC2, hyperactive MAPK/ERK signaling, and Noonan-like syndrome (3). Alternate Names FLJ60412; KIAA0862; Leucine-rich repeat protein SHOC-2; NSLH1; Protein soc-2 homolog; Protein sur-8 homolog; SHOC2; SHOC2 leucine rich repeat scaffold protein; SIAA0862; SOC-2; soc-2 suppressor of clear homolog; soc-2 suppressor of clear homolog (C. elegans); SOC2; suppressor of clear, C. elegans, homolog of; SUR-8; SUR8 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 64 Source/Isotype: Rabbit IgG