CST, 53626S, MAP7 (E8K7O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MAP7. Validated for Western Blotting,Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, MAP7 (E8K7O) Rabbit Monoclonal Antibody (CST #53626) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Frozen): 1:800 - 1:3200 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity MAP7 (E8K7O) Rabbit Monoclonal Antibody recognizes endogenous levels of total MAP7 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MAP7 protein. Background MAP7 is a member of the Microtubule-Associated Proteins (MAPs) that regulates the stability and function of the cytoskeleton in cells (1). MAP7 facilitates the transport of organelles, such as mitochondria and lysosomes, via recruitment of kinesin-1 (2). This role of MAP7 in organelle transport occurs in neuronal and non-neuronal cells, including oocytes, and in S2 cells and mammalian muscle cells (4,5). In addition to promoting organelle transport via kinesin-1 recruitment, MAP7 also contributes to the morphogenesis of axons that is domain-specific, whereas the full-length MAP7 promotes branch maturation, N- and P- domains are responsible for branch formation, and the C-terminal kinesin-interacting domain contributes to the axonal growth (3,6). MAP7 and the paralog MAP7D1 directly interact with disheveled, an effector of the Wnt5a β-catenin-independent pathway, regulating the microtubule remodeling, cell migration, and cell adhesion in HeLa cells (7). MAP7 is considered a biomarker in a model to treat multiple sclerosis based on the tolerogenic dendritic cells induced by vitamin D3 (8). Alternate Names dJ325F22.2 (microtubule-associated protein 7 (EMAP115, E-MAP-115)); E-MAP-115; EMAP115; Ensconsin; Epithelial microtubule-associated protein of 115 kDa; MAP-7; MAP7; microtubule associated protein 7; Microtubule-associated protein 7 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 110 Source/Isotype: Rabbit IgG