CST, 53668T, HSP10 (F8X5Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying HSP10. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, HSP10 (F8X5Y) Rabbit Monoclonal Antibody (CST #53668) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity HSP10 (F8X5Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total HSP10 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human HSP10 protein. Background Impaired mitochondrial integrity and function lead to the activation of a transcriptional response known as the mitochondrial unfolded protein response (UPR ) (1). Mitochondrial 10 kDa heat shock protein (HSP10), also known as heat shock protein family E member 1 (HSPE1), is a mitochondria-specific protein chaperone and a component in the UPR pathway. Studies show that Akt3 depletion in primary endothelial cells causes mitochondrial stress and leads to upregulated expression of HSP10 along with HSP60, another mitochondria-specific protein chaperone. This finding suggests a critical role of Akt3 in maintaining mitochondrial homeostasis (2). In addition, HSP10 and HSP60 chaperones were shown to be the most abundant mitochondrial proteins (3). Alternate Names 10 kDa chaperonin; 10 kDa heat shock protein, mitochondrial; CH10; Chaperonin 10; CPN10; Early-pregnancy factor; EPF; epididymis secretory sperm binding protein; GROES; heat shock 10kD protein 1 (chaperonin 10); heat shock 10kDa protein 1; heat shock 10kDa protein 1 (chaperonin 10); heat shock protein family E (Hsp10) member 1; Hsp10; HSPE1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 10 Source/Isotype: Rabbit IgG