CST, 53674S, STAM2 (D8B3G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying STAM2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, STAM2 (D8B3G) Rabbit Monoclonal Antibody (CST #53674) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity STAM2 (D8B3G) Rabbit Monoclonal Antibody recognizes endogenous levels of total STAM2 protein. This antibody does not cross-react with STAM1 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human STAM2 protein. Background Signal transducing adaptor molecule 2 (STAM2) is a ubiquitously expressed STAM family adaptor protein and an integral component of the ESCRT-0 complex. Similar to STAM1, STAM2 possesses a single SH3 domain and an immunoreceptor tyrosine-based activation motif (ITAM). Following activation of multiple growth factor and cytokine cell surface receptors, the STAM2 protein undergoes tyrosine phosphorylation and potentiates mitogenic signals driven by these receptors (1,2). Research studies demonstrate that STAM2 is localized to complexes containing Eps15, Hrs, and STAM1 proteins on early endosome membranes. A tandem, amino-terminal VHS (Vps27/Hrs/STAM) domain and UIM (ubiquitin-interacting) motif within STAM2 facilitate STAM2 interaction with ubiquitinated cargo proteins, suggesting that this adaptor participates in the endosomal sorting of ubiquitinated proteins targeted for lysosomal degradation (3-6). Gene targeting studies have revealed an indispensible role for STAM2 in T-cell development (7). Alternate Names DKFZp564C047; HBP; Hrs-binding protein; HSE1 homolog; Signal transducing adapter molecule 2; signal transducing adaptor molecule (SH3 domain and ITAM motif) 2; signal transducing adaptor molecule 2; STAM-2; STAM-like protein containing SH3 and ITAM domains 2; STAM2; STAM2A; STAM2B Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 70 Source/Isotype: Rabbit IgG