CST, 53753SF, T-bet/TBX21 (E4I2K) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying TBX21 mouse. Validated for WB,IHC,IF,F. Highly specific and rigorously validated in-house, T-bet/TBX21 (E4I2K) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #53753) is ready to ship. Product Usage Information This product is the carrier-free version of product #97135. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS, BSA and Azide free. For standard formulation of this product see product # 97135 . Storage Store at -20ºC. This product will freeze at -20ºC so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity T-bet/TBX21 (E4I2K) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total T-bet/TBX21 protein. E412K non-specifically labels the submucosa and external muscularis of mouse colon by immunofluorescence. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment derived from mouse T-bet/TBX21 protein. Background The gene family consists of transcription factors characterized by a related DNA-binding domain (T-box) of approximately 200 amino acids (1,2). The genes exhibit diverse temporal and spatial patterns in the developing embryo. Studies have demonstrated members of this family play crucial roles during embryogenesis in a wide range of organisms by regulating cell fate decisions to establish the early body plan and to regulate later processes underlying organogenesis (3-5). Mutations in genes are associated with many developmental defects (6). Recent studies also indicate potential roles in cancer by members of the T-box family (7-9). Alternate Names OTTMUSP00000002150; T-bet; T-box 21; T-box protein 21; T-box transcription factor TBX21; T-cell-specific T-box transcription factor T-bet; Tbet; Tbly; Tblym; TBT1; Tbx21; Transcription factor TBLYM Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 62 Source/Isotype: Rabbit IgG