CST, 5378S, Msx1 (P5) Antibody

Polyclonal Antibody for studying MSX1. Validated for Western Blotting. Highly specific and rigorously validated in-house, Msx1 (P5) Antibody (CST #5378) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Msx1 (P5) Antibody detects endogenous levels of total Msx1 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acid sequences surrounding Pro5 of human Msx1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Msh homeobox 1 (Msx1) is a Muscle Segment Homeobox (Msh) gene family member that acts as a transcriptional repressor during embryonic development, playing an important role in limb pattern formation, craniofacial development, and tooth development (1-3). Msx1 is expressed in the mesenchyme of the developing nail bed (2) and in fetal hair follicles, epidermis and fibroblasts; reduced expression is seen in adult epithelial-derived tissues (4). Msx1 acts in concert with the Wnt1 network to establish the midbrain dopaminergic progenator domain, a region that gives rise to neurons that are critical for normal brain function and are the cells affected in Parkinson disease (5). Mutation in the corresponding Msx1 gene correlates with abnormal tooth development in patients diagnosed with Wolf-Hirschhorn syndrome (6). Other genetic changes in the Msx1 gene result in Witkop Syndrome ("tooth and nail syndrome") and cases of abnormal tooth development associated with non-syndromic orofacial clefting (2,7). Alternate Names ECTD3; homeobox 7; Homeobox protein Hox-7; Homeobox protein MSX-1; HOX7; HYD1; msh homeo box 1; msh homeobox 1; Msh homeobox 1-like protein; msh homeobox homolog 1; MSX1; STHAG1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 32 SOURCE: Rabbit