CST, 5380S, hnRNP A1 (R196) Antibody

Polyclonal Antibody for studying hnRNP A1. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, hnRNP A1 (R196) Antibody (CST #5380) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity hnRNP A1 (R196) Antibody detects both isoforms of endogenous hnRNP A1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg196 of human hnRNP A1 protein. Antibodies were purified by protein A and peptide affinity chromatography. Background Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) is a member of the hnRNP A/B family of related RNA binding proteins that bind pre-mRNA and are involved in the processing, metabolism, and transport of nuclear pre-mRNA transcripts (1). hnRNP A1 regulates the alternative splicing of c-Src and c-H-Ras (2,3) and modifies initiation of translation of the fibroblast growth factor 2 mRNA (4). hnRNP A1 expression level is elevated in many cancers; knockdown of hnRNP A1 leads to apoptosis in various cancer cells (5). Although predominantly nuclear, hnRNP A1 is continually transported from the nucleus to the cytoplasm where it disassociates from mRNA and is rapidly re-imported into the nucleus (6,7). hnRNP A1 binds to cis-acting repressive sequences (CRS) of HIV-1 to influence HIV-1 production (8,9). HIV-1 enhances hnRNP A1 expression and promotes the relocalization of hnRNP A1 to the cytoplasm (10). Alternate Names ALS19; ALS20; epididymis secretory sperm binding protein; Helix-destabilizing protein; Heterogeneous nuclear ribonucleoprotein A1; Heterogeneous nuclear ribonucleoprotein A1, N-terminally processed; heterogeneous nuclear ribonucleoprotein A1B protein; heterogeneous nuclear ribonucleoprotein B2 protein; heterogeneous nuclear ribonucleoprotein core protein A1; hnRNP A1; hnRNP core protein A1; hnRNP core protein A1-like 3; hnRNP-A1; HNRNPA1; HNRPA1; HNRPA1L3; IBMPFD3; MGC102835; nuclear ribonucleoprotein particle A1 protein; putative heterogeneous nuclear ribonucleoprotein A1-like 3; ROA1; single-strand DNA-binding protein UP1; Single-strand RNA-binding protein; UP 1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 34, 40 SOURCE: Rabbit