CST, 53842T, TIPE2 (E5Q2S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TIPE2. Validated for WB,IP,IF,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, TIPE2 (E5Q2S) Rabbit Monoclonal Antibody (CST #53842) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Frozen): 1:400 - 1:800 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Flow Cytometry (Fixed/Permeabilized): 1:800 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity TIPE2 (E5Q2S) Rabbit Monoclonal Antibody recognizes endogenous levels of total TIPE2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the sequence of human TIPE2 protein, including the carboxy terminus. Background Tumor necrosis factor-α (TNF-α)-induced protein 8-like 2 (TIPE2) is a negative regulator of innate and adaptive immunity, and is important in maintaining immune homeostasis. TIPE2 inhibits TGF-β activated kinase 1 (TAK1), a key regulator of inflammatory signaling (1). TIPE2 inhibits oncogenic Ras activity, and deficiency of TIPE2 is associated with resistance to cell death and increased cell migration (2). TIPE2 is downregulated in several human cancers, and may serve as a tumor suppressor and biomarker (3). Alternate Names FLJ23467; inflammation factor 20; Inflammation factor protein 20; OTTHUMP00000014152; Q6P589; TIPE2; TNF alpha induced protein 8 like 2; TNF alpha-induced protein 8-like protein 2; TNFAIP8-like protein 2; TNFAIP8L2; TP8L2; Tumor necrosis factor alpha-induced protein 8-like protein 2; tumor necrosis factor, alpha induced protein 8 like 2; tumor necrosis factor, alpha-induced protein 8-like 2; tumor necrosis factor, alpha-induced protein 8-like protein 2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 20 Source/Isotype: Rabbit IgG