CST, 53867SF, STEAP1 (D8B2V) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying STEAP1. Validated for Western Blotting. Highly specific and rigorously validated in-house, STEAP1 (D8B2V) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #53867) is ready to ship. Product Usage Information This product is the carrier free version of product #88677. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 88677 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity STEAP1 (D8B2V) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total STEAP1 protein. This antibody also recognizes overexpressed STEAP1B isoform 1 protein and may recognize endogenous STEAP1B isoform 1 protein. Species Reactivity: Human, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human STEAP1 protein. Background Six transmembrane epithelial antigen of the prostate 1 (STEAP1) is a transmembrane protein abundantly expressed in normal prostate epithelial cells (1). It is a metalloreductase that reduces Fe to Fe and Cu to Cu (2). STEAP1 is also reportedly overexpressed in multiple tumor types, and has been used as a cell surface biomarker for prostate cancer and Ewing's sarcoma (3-6). Peptides derived from STEAP1 protein have been shown to induce an antigen-specific cytotoxic T lymphocyte (CTL) response that can kill tumor cells expressing STEAP1 (7). These finding are the basis for research studies examining the efficacy of antibody-drug conjugates (ADCs) targeting STEAP1 (8,9). Alternate Names Metalloreductase STEAP1; MGC19484; PRSS24; six transmembrane epithelial antigen of the prostate 1; Six-transmembrane epithelial antigen of prostate 1; STEA1; STEAP; STEAP family member 1; STEAP1; STEAP1 metalloreductase; STEAP1 protein Specification REACTIVITY: H R SENSITIVITY: Endogenous MW (kDa): 30 Source/Isotype: Rabbit IgG