CST, 53886S, SPEN/SHARP (F6J4M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SPEN. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, SPEN/SHARP (F6J4M) Rabbit Monoclonal Antibody (CST #53886) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SPEN/SHARP (F6J4M) Rabbit Monoclonal Antibody recognizes endogenous levels of total SPEN/SHARP protein. This antibody detects a 300 kDa protein and a 500 kDa protein of unknown identities in some cell lines. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human SPEN/SHARP protein. Background Msx2-interacting protein (SPEN or split ends), also known as silencing mediator for retinoid or thyroid hormone receptors (SMRT)/histone deacetylases (HDACs)-associated repressor protein (SHARP), is a potent transcriptional repressor that contains four highly conserved RNA recognition motifs (RRMs) at its N-terminus and an SPOC domain within its C-terminus that is responsible for protein-protein associations with repressive complexes (1,2). SPEN/SHARP has been studied in the context of various cell signaling events, such as the NOTCH or nuclear hormone receptor signaling pathways, and is also critical in the X chromosome inactivation (XCI) process (3-6). More specifically, this protein functions through interacting with X inactive specific transcript (XIST), a long noncoding RNA that primarily functions to inactivate one X chromosome in female cells to achieve dosage compensation. This is achieved by recruiting various repressor complex members, such as SMRT, nuclear receptor corepressor (NCoR), and nucleosome remodeling deacetylase (NuRD) complexes (4-9). SPEN/SHARP has been reported as a novel tumor suppressor that may be clinically utilized as a biomarker for tamoxifen response in ERα-positive breast cancers (10) and has also been shown to be required for various developmental processes (11). Alternate Names HIAA0929; KIAA0929; MINT; Msx2 interacting nuclear target (MINT) homolog; Msx2-interacting protein; nuclear receptor transcription cofactor; RBM15C; RP1-134O19.1; SHARP; SMART/HDAC1 associated repressor protein; SMART/HDAC1-associated repressor protein; SPEN; spen family transcriptional repressor; SPEN homolog; spen homolog, transcriptional regulator; spen homolog, transcriptional regulator (Drosophila) Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 402 Source/Isotype: Rabbit IgG