CST, 5388S, RXR alpha Antibody

Polyclonal Antibody for studying RXR-alpha. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, RXR alpha Antibody (CST #5388) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity RXR alpha Antibody recognizes endogenous levels of total RXRα protein. This antibody does not cross-react with either RXRβ or RXRγ. Species Reactivity: Human, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human RXRα protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The human retinoid X receptors (RXRs) are encoded by three distinct genes ( α, β, and γ) and bind selectively and with high affinity to the vitamin A derivative, 9- retinoic acid. RXRs are type-II nuclear hormone receptors that are primarily localized to the nuclear compartment independent of ligand binding. Nuclear RXRs form heterodimers with nuclear hormone receptor subfamily 1 proteins, including thyroid hormone receptor, retinoic acid receptors, vitamin D receptor, peroxisome proliferator-activated receptors, liver X receptors, and farnesoid X receptor (1). Since RXRs heterodimerize with multiple nuclear hormone receptors, they play a central role in transcriptional control of numerous hormonal signaling pathways by binding to -acting response elements in the promoter/enhancer region of target genes (2). Retinoid X receptor α (RXRα) is the founding RXR family member and is predominantly expressed in liver, kidney, epidermis, intestine and a variety of tissues (2-4). Knockout of the murine α gene results in embryonic lethality tentatively due to myocardial hypoplasia, which demonstrates the importance of RXRα to retinoid signaling (5,6). Biochemical evidence suggests that RXRα transcriptional activity is post-translationally regulated through the Ras-Raf-MAPK signaling cascade. MAPK-dependent phosphorylation of RXRα directly abrogates the ability of RXRα to associate with nuclear receptor coactivators (7). Alternate Names FLJ00280; FLJ00318; FLJ16020; FLJ16733; MGC102720; NR2B1; Nuclear receptor subfamily 2 group B member 1; Retinoic acid receptor RXR-alpha; retinoid X nuclear receptor alpha; Retinoid X receptor alpha; retinoid X receptor, alpha; RXR-alpha; RXRA Specification REACTIVITY: H R Mk SENSITIVITY: Endogenous MW (kDa): 53 SOURCE: Rabbit