CST, 5389T, NME1/NDKA (D18F10) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NME1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, NME1/NDKA (D18F10) Rabbit Monoclonal Antibody (CST #5389) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity NME1/NDKA (D18F10) Rabbit Monoclonal Antibody detects endogenous levels of total NME1/NDKA protein. This antibody is predicted to cross-react with NME2/NDKB protein. Species Reactivity: Human, Mouse, Rat, Monkey, Bovine Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to a region surrounding Pro96 of human NME1/NDKA protein. Background The NDK/NME/NM23 kinase family (encoded by the gene family) consists of at least eight distinct proteins that exhibit different cellular localization (1). Members of this group inhibit metastasis in a variety of tumor cell types (2). All NDK/NME/NM23 proteins possess nucleoside diphosphatase kinase (NDK) activity and catalyze the phosphorylation of nucleoside diphosphate to the corresponding nucleoside triphosphate to regulate a diverse array of cellular events (3). At least four classes of NDK biochemical activities have been described, including protein-protein interactions (4-6), regulation of GTP-binding protein function (7-9), DNA-associated activities (10,11), and histidine-dependent protein phosphotransferase activity (12). NDK/NME proteins participate in the regulation of a broad spectrum of cellular responses, including development, differentiation, proliferation, endocytosis, and apoptosis (13). Because of its role in metastasis suppression and oncogenesis, NDKA (NME1/NM23-H1) has been widely studied (14). NDKA (NM23-H1) and NDKB (NM23-H2) are encoded by adjacent and genes and share 90% sequence identity. Two serine residues (Ser122 and Ser144) on NDKA/NM23-H1 can be phosphorylated by AMPKα1, but only phosphorylation at Ser122 determines whether NDKA channels ATP to AMPKα1. This regulates AMPKα1 activity towards ACC1, an important regulator of fatty acid metabolism (15). Mutation of NDKB/NM23-H2 at Ser122 (S122P) in melanoma cells results in altered phosphoryl transfer activity (16). Alternate Names AWD; epididymis secretory sperm binding protein; GAAD; Granzyme A-activated DNase; Metastasis inhibition factor nm23; NB; NBS; NDK A; NDKA; NDP kinase A; NDPK-A; NDPKA; NM23; nm23-H1; NME/NM23 nucleoside diphosphate kinase 1; NME1; NME1A; non-metastatic cells 1, protein (NM23A) expressed in; Nucleoside diphosphate kinase A; Tumor metastatic process-associated protein Specification REACTIVITY: H M R Mk B SENSITIVITY: Endogenous MW (kDa): 16, 18 Source/Isotype: Rabbit IgG