CST, 53957S, IL-1 alpha (D4F3S) Rabbit Monoclonal Antibody (PE Conjugate)

Monoclonal Antibody for studying IL1 alpha mouse. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, IL-1 alpha (D4F3S) Rabbit Monoclonal Antibody (PE Conjugate) (CST #53957) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity IL-1 alpha (D4F3S) Rabbit Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of total IL-1α protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of mouse IL-1α protein. Background Interleukin 1 alpha (IL-1α) belongs to the IL-1 family of cytokines with 11 members including IL-1β. IL-1α is expressed in many cell types of both hematopoietic and non-hematopoietic origins under steady state, and its expression can be increased in response to appropriate stimuli (1,2). Like IL-1β, IL-1α is also synthesized as a precursor (pro-IL-1α) and can be cleaved into smaller mature forms. However, both pro-IL-1α and the cleaved form of IL-1α are biologically active and can activate the signaling pathway through the membrane receptor IL-1R1. IL-1α is active both as a secreted form and as a membrane-bound form. Due to such characteristics, passive leakage of IL-1α from dying cells can activate inflammation, leading some researchers to consider IL-1α as a key "alarmin in the cell" that alerts the host to damage or injury (3,4). In addition, IL-1α can also enter the nucleus to modulate transcription (5,6). Alternate Names IL-1 alpha; Il-1a; Il1a; interleukin 1 alpha; Interleukin-1 alpha; OTTMUSP00000016430 Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG